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one hybrid library screening system  (TaKaRa)


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    Structured Review

    TaKaRa one hybrid library screening system
    One Hybrid Library Screening System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/screening+libraries/Matchmaker+Gold+Yeast+One-Hybrid+Library+Screening+System/10__1016_slash_j__indcrop__2026__123372-72-13-17
    Average 98 stars, based on 1235 article reviews
    one hybrid library screening system - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. Screening of a 614 cDNA library was performed with the Matchmaker Gold Y1H Library Screening System (Cat. 615 No. 630491; Takara, Kyoto, Japan) with stringent selection with 40 ng/mL AbA (AbA; 616 Coolaber, Beijing, China). ..

    Library Screening:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. Screening of a 614 cDNA library was performed with the Matchmaker Gold Y1H Library Screening System (Cat. 615 No. 630491; Takara, Kyoto, Japan) with stringent selection with 40 ng/mL AbA (AbA; 616 Coolaber, Beijing, China). ..

    Article Title: ABA-independent PP2C-binding in PYLs traces to bacterial origins and persists in land plants.
    Article Snippet: Following incubation, the samples were decolorized with 75% and 95% ethanol and water, subsequently at 37 °C and finally imaged by a Stemi 305 stereomicroscope (Zeiss). .. Yeast one-hybrid assays were conducted using the Yeast OneHybrid Library Screening System (Clontech), following the manufacturer’s instructions. ..

    Article Title: VvWRKY70, a newly identified grape WRKY transcription factor, confers drought tolerance via coordinated physiological and molecular responses.
    Article Snippet: Drought is a key factor limiting grape yield, making it crucial to reveal the underlying response mechanisms of plants to environmental stress.. Although WRKY transcription factors play multiple roles in plant development and stress responses, only a few have been functionally characterized in Vitis vinifera L... In this study, VvWRKY70, from V. vinifera, was cloned and identified in transgenic A. thaliana plants and grape seedlings.

    Article Title: MhORG2, a novel negative modulator, regulates drought tolerance in apple by repressing the expression MhAAO3 and antioxidant enzyme genes.
    Article Snippet: Drought stress severely impacts the growth, yield, and quality of apple (Malus domestica).. Abscisic acid (ABA) and basic helix-loop-helix (bHLH) transcription factors play crucial roles in regulating the drought response in many plants, but the potential interactions between bHLH and ABA in response to drought in apple still need to be discovered.. Herein, we identified a bHLH transcription factor, ORG2 (OBP3‐responsive gene 2), from M. hupehensis, and the expression of which is induced by drought and ABA.

    Article Title: Identification of Target Genes of Transcription Factor DpAP2 Related to Carotenoid Biosynthesis from Dunaliella Parva.
    Article Snippet: Carotenoids act as the precursors of vitamin A and natural antioxidants, which benefit human health.. The APETALA2/Ethylene Responsive Factor (AP2/ERF), as a transcription factor family, involved in various biological processes of higher plants, such as playing a key role in regulation of effective carotenoid accumulation.. Several reports showed that plant′s AP2/ERF transcription factors (TFs) could response to various stresses, plant morphogenesis, hormone signal transduction and metabolite regulation.

    Article Title: The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.
    Article Snippet: .. The CDS sequence of NnABI5 was cloned into the pGBKT7 vector, and the resulting pGBKT7-NnABI5 recombinant plasmid was transformed into the Matchmaker Gold Y2H Library Screening System (Clontech, Dalian, China) for Y2H assays. ..

    Article Title: Transcription factor SmMYC2 regulates secondary cell wall thickening to enhance drought resilience in eggplant.
    Article Snippet: Key message SmMYC2 promotes SmNST1 expression and inhibits the expression of SmMYB108 and SmMYC2 itself in vivo, then thickens secondary cell wall and enhances drought resilience of eggplant.. Abstract As climate change worsens, drought stress has emerged as a critical limiting factor for crop productivity.. Members of the basic helix–loop–helix (bHLH) transcription factor family exhibit multifunctional regulatory roles in plant adaptation mechanisms.

    Selection:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. Screening of a 614 cDNA library was performed with the Matchmaker Gold Y1H Library Screening System (Cat. 615 No. 630491; Takara, Kyoto, Japan) with stringent selection with 40 ng/mL AbA (AbA; 616 Coolaber, Beijing, China). ..

    Activity Assay:

    Article Title: Identification of Target Genes of Transcription Factor DpAP2 Related to Carotenoid Biosynthesis from Dunaliella Parva.
    Article Snippet: Carotenoids act as the precursors of vitamin A and natural antioxidants, which benefit human health.. The APETALA2/Ethylene Responsive Factor (AP2/ERF), as a transcription factor family, involved in various biological processes of higher plants, such as playing a key role in regulation of effective carotenoid accumulation.. Several reports showed that plant′s AP2/ERF transcription factors (TFs) could response to various stresses, plant morphogenesis, hormone signal transduction and metabolite regulation.

    Chromatin Immunoprecipitation:

    Article Title: Identification of Target Genes of Transcription Factor DpAP2 Related to Carotenoid Biosynthesis from Dunaliella Parva.
    Article Snippet: Carotenoids act as the precursors of vitamin A and natural antioxidants, which benefit human health.. The APETALA2/Ethylene Responsive Factor (AP2/ERF), as a transcription factor family, involved in various biological processes of higher plants, such as playing a key role in regulation of effective carotenoid accumulation.. Several reports showed that plant′s AP2/ERF transcription factors (TFs) could response to various stresses, plant morphogenesis, hormone signal transduction and metabolite regulation.

    Sequencing:

    Article Title: The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.
    Article Snippet: .. The CDS sequence of NnABI5 was cloned into the pGBKT7 vector, and the resulting pGBKT7-NnABI5 recombinant plasmid was transformed into the Matchmaker Gold Y2H Library Screening System (Clontech, Dalian, China) for Y2H assays. ..

    Clone Assay:

    Article Title: The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.
    Article Snippet: .. The CDS sequence of NnABI5 was cloned into the pGBKT7 vector, and the resulting pGBKT7-NnABI5 recombinant plasmid was transformed into the Matchmaker Gold Y2H Library Screening System (Clontech, Dalian, China) for Y2H assays. ..

    Plasmid Preparation:

    Article Title: The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.
    Article Snippet: .. The CDS sequence of NnABI5 was cloned into the pGBKT7 vector, and the resulting pGBKT7-NnABI5 recombinant plasmid was transformed into the Matchmaker Gold Y2H Library Screening System (Clontech, Dalian, China) for Y2H assays. ..

    Recombinant:

    Article Title: The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.
    Article Snippet: .. The CDS sequence of NnABI5 was cloned into the pGBKT7 vector, and the resulting pGBKT7-NnABI5 recombinant plasmid was transformed into the Matchmaker Gold Y2H Library Screening System (Clontech, Dalian, China) for Y2H assays. ..

    Transformation Assay:

    Article Title: The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.
    Article Snippet: .. The CDS sequence of NnABI5 was cloned into the pGBKT7 vector, and the resulting pGBKT7-NnABI5 recombinant plasmid was transformed into the Matchmaker Gold Y2H Library Screening System (Clontech, Dalian, China) for Y2H assays. ..

    Transfection:

    Article Title: Transcription factor SmMYC2 regulates secondary cell wall thickening to enhance drought resilience in eggplant.
    Article Snippet: Key message SmMYC2 promotes SmNST1 expression and inhibits the expression of SmMYB108 and SmMYC2 itself in vivo, then thickens secondary cell wall and enhances drought resilience of eggplant.. Abstract As climate change worsens, drought stress has emerged as a critical limiting factor for crop productivity.. Members of the basic helix–loop–helix (bHLH) transcription factor family exhibit multifunctional regulatory roles in plant adaptation mechanisms.

    Cell Culture:

    Article Title: Transcription factor SmMYC2 regulates secondary cell wall thickening to enhance drought resilience in eggplant.
    Article Snippet: Key message SmMYC2 promotes SmNST1 expression and inhibits the expression of SmMYB108 and SmMYC2 itself in vivo, then thickens secondary cell wall and enhances drought resilience of eggplant.. Abstract As climate change worsens, drought stress has emerged as a critical limiting factor for crop productivity.. Members of the basic helix–loop–helix (bHLH) transcription factor family exhibit multifunctional regulatory roles in plant adaptation mechanisms.

    Synthesized:

    Article Title: Identification of villin-interacting virulence factors of Blastocystis hominis using yeast two-hybrid screening.
    Article Snippet: Blastocystis hominis (B. hominis), one of the most common intestinal protozoa infecting humans worldwide, is associated with gastrointestinal disorders, yet it remains unclear whether it secretes virulence factors that interact with villin, a key cytoskeletal protein essential for brush border homeostasis and epithelial barrier function.. In this study, we constructed a high-quality normalized full-length B. hominis cDNA library (capacity 5.2 × 106 CFU; recombination rate 95.8%) and performed yeast two-hybrid screening using villin as bait.. Thirty initial positive clones were obtained, and sequencing combined with BLAST identified 24 unique prey proteins.



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